Protocol Hub
Evidence-graded stacking protocols. Each protocol lists the compounds used, their daily schedule, and the evidence behind the combination.
The Athletic Longevity Protocol — Creatine, Beta-Alanine, Citrulline & Taurine
A four-compound performance stack that covers each phase of high-intensity effort through a distinct mechanism: creatine fuels maximal short bursts, beta-alanine buffers the acidosis of 1–4 minute efforts, citrulline raises nitric oxide for blood flow and ammonia clearance, and taurine supports cellular function with a longevity angle. Three are dosed daily for accumulation; citrulline is timed pre-workout. Built on amino acids and energy substrates with strong safety profiles, for athletes who want mechanism-matched stacking rather than single-ingredient hype.
4 steps · 4 compounds
View protocol →The Bio-Clock Reset Protocol
The most comprehensive biological clock reset protocol available outside clinical settings. Combines Professor Khavinson's original dual-peptide longevity protocol (Epithalon + Thymalin) with Magnesium L-Threonate for sleep and pineal support. Targets the two master regulators of biological ageing - the neuroendocrine system and the immune system - simultaneously, in the tradition of the landmark St. Petersburg longevity research.
3 steps · 3 compounds
View protocol →Cognitive Peak Protocol
Five-compound cognitive enhancement and neuroprotection stack combining Creatine, Alpha-GPC, Lion's Mane, Magnesium Threonate, and Lithium Orotate — targeting energy metabolism, acetylcholine synthesis, neurogenesis, synaptic density, and GSK-3β neuroprotection.
5 steps · 5 compounds
View protocol →Comprehensive Senolytic Protocol 2026
The most advanced 2026 senolytic protocol combining three mechanistically distinct approaches: direct senolysis (Quercetin Phytosome pulsed), natural flavonoid senolysis (Fisetin pulsed), and indirect immune-mediated clearance (SGLT2 inhibitor) — with Urolithin A supporting immune function. The complete protocol for advanced longevity-focused individuals.
4 steps · 4 compounds
View protocol →Deep Sleep Architect
A research-backed evening protocol targeting both sides of the sleep equation: cortisol reduction and brain magnesium optimisation. Ashwagandha KSM-66 lowers the stress hormone that delays sleep onset; Magnesium L-Threonate crosses the blood-brain barrier to improve sleep architecture and REM quality. Suitable for anyone with elevated evening stress, poor sleep quality, or frequent night waking.
2 steps · 2 compounds
View protocol →Deep Sleep Stack
A four-compound layered sleep protocol targeting four independent mechanisms — brain magnesium (magnesium L-threonate), GABA-A modulation (apigenin), alpha-wave calm and reduced arousal (L-theanine), and core-temperature drop (glycine). Built for sleep onset and depth without sedative-hypnotic dependence.
4 steps · 4 compounds
View protocol →The Dopamine Reset Stack — 9-Me-BC Protocol
A protocol for restoring dopaminergic function after stimulant overuse, burnout, or receptor downregulation. 9-Me-BC promotes dopaminergic neuron proliferation and inhibits MAO-B; Lion's Mane supports neuroplasticity; Selank manages stimulatory side effects. For experienced biohackers only.
3 steps · 3 compounds
View protocol →Female Hormonal Optimization Protocol
A conservative, bloodwork-guided protocol that restores the female steroid cascade from the top: pregnenolone refills the precursor pool, DHEA restores the key downstream androgen at a female-specific low dose, and maca supports libido and energy through non-hormonal pathways. Built for perimenopausal and postmenopausal women working with a clinician.
3 steps · 3 compounds
View protocol →The Female Longevity Stack
Evidence-based longevity protocol designed specifically for women's healthspan — addressing hormonal fluctuations, bone density, cardiovascular protection, and cognitive preservation across the lifespan.
6 steps · 6 compounds
View protocol →The Flow State Stack
A precision cognitive performance stack combining Russia's two most studied nootropic peptides with a cholinergic foundation. Semax provides dopaminergic drive and BDNF-mediated neuroplasticity; Selank provides GABAergic calm and anxiety reduction; Alpha-GPC provides the acetylcholine substrate both peptides work on. The result is a sustained, clear-headed flow state without stimulant side effects - designed for knowledge workers, creatives, and high performers.
3 steps · 3 compounds
View protocol →Foundational Longevity Stack
The most widely adopted evidence-based longevity stack, built around NAD+ restoration and sirtuin pathway activation. Suitable for adults over 35 seeking a science-backed starting point for longevity supplementation.
3 steps · 3 compounds
View protocol →Lean GLP-1 Protocol
A muscle-preservation protocol for GLP-1 and dual-agonist users. Pairs the incretin drug (dosed for body composition, not speed) with creatine and an optional off-cycle berberine bridge — alongside the non-pharmacological pillars of high protein and resistance training that defend lean mass during rapid fat loss.
3 steps · 3 compounds
View protocol →The Gut Barrier Protocol — BPC-157 + KPV & Advanced Additions
A comprehensive gut barrier restoration protocol with two tiers. Core Protocol: BPC-157 (mucosal regeneration via VEGF) and KPV (inflammation suppression via MC1R/NF-kB). Advanced Additions: Colostrum (growth factor-mediated regeneration), Sodium Butyrate (colonocyte energy and epigenetic modulation), and Zinc (tight junction cofactor). Designed for leaky gut, IBD, post-antibiotic dysbiosis, and SIBO recovery.
7 steps · 5 compounds
View protocol →Hormonal Optimization Stack — Foundation & Extended
A two-tier natural testosterone optimisation stack. Foundation: Tongkat Ali (SHBG reduction + LH support) and Ashwagandha (cortisol modulation). Extended Stack adds Fadogia Agrestis (LH stimulation), Boron (SHBG reduction), and Zinc (testosterone synthesis cofactor). Targets both production and suppression of testosterone — the most evidence-backed supplement combination for hormonal balance in men over 35.
5 steps · 5 compounds
View protocol →The Lean Mass Preservation Stack — GLP-1 + Growth Hormone Protocol
A protocol combining a GLP-1/GIP/glucagon triple agonist (retatrutide) with growth hormone secretagogues (ipamorelin + CJC-1295) to achieve aggressive fat loss while preserving lean mass. Addresses the primary limitation of GLP-1 therapy: muscle catabolism during caloric restriction.
3 steps · 3 compounds
View protocol →Metabolic Longevity Protocol
Advanced metabolic optimisation combining Semaglutide, Berberine, Acarbose, and NMN — targeting body composition, insulin sensitivity, glucose metabolism, and NAD+ restoration for comprehensive metabolic longevity.
4 steps · 4 compounds
View protocol →The Metabolic Optimizer
An advanced metabolic reprogramming protocol for practitioners with significant biohacking experience. Combines mitochondrial DNA signalling (MOTS-c), direct AMPK activation (AICAR), and NAD+ restoration (NMN) to comprehensively address cellular energy metabolism. Targets the same pathways as caloric restriction and endurance exercise at the molecular level. Requires blood glucose monitoring and is not appropriate for beginners.
3 steps · 3 compounds
View protocol →The Ultimate Mitochondrial Matrix — SS-31 + MOTS-c + NAD+ & Advanced Tier
A comprehensive mitochondrial restoration protocol with two tiers. Foundation Tier: SS-31 (inner membrane repair), MOTS-c (biogenesis activation), NMN (NAD+ substrate), PQQ (PGC-1α stimulation). Advanced Tier adds Urolithin A (mitophagy) and Taurine (membrane stability). Designed for chronic fatigue, exercise performance decline, and mitochondrial age-related dysfunction.
6 steps · 6 compounds
View protocol →The mTOR Sabbatical — Rapamycin + Acarbose + Berberine Protocol
A two-tier longevity protocol centred on pulsed mTOR inhibition. Foundation Tier: weekly rapamycin with daily NMN and spermidine for autophagy support. Advanced Tier adds acarbose (postprandial glucose control) and berberine (AMPK activation) for three-pathway mTOR inhibition. The ITP showed rapamycin + acarbose produced 28% median lifespan extension in mice. Requires physician prescription and monitoring.
5 steps · 5 compounds
View protocol →Neuro-Regen Stack — Long-Term Brain Optimisation
A three-compound protocol targeting neuroregeneration and long-term cognitive performance. Lion's Mane stimulates NGF and new neural connections; Bacopa Monnieri consolidates memory and strengthens existing synapses; Alpha-GPC provides the acetylcholine substrate for both. Designed for knowledge workers, high performers, and anyone seeking neuroprotection against age-related cognitive decline.
3 steps · 3 compounds
View protocol →Neuroprotection & Alzheimer's Prevention Protocol
Four-compound Alzheimer's prevention stack targeting GSK-3β (Lithium Orotate), neuroinflammation (Omega-3 DHA), NGF/BDNF stimulation (Lion's Mane), and synaptic density (Magnesium Threonate) — for individuals with genetic risk or cognitive decline concerns.
4 steps · 4 compounds
View protocol →The Sexual Wellness Protocol
A three-layer protocol addressing sexual wellness from the ground up: Zinc builds the hormonal foundation; PT-141 activates central arousal pathways via melanocortin receptors; Oxytocin deepens emotional connection and amplifies the bonding dimension of intimacy. The only evidence-backed supplement stack that addresses both the physiological and psychological dimensions of sexual function simultaneously.
3 steps · 3 compounds
View protocol →SGLT2 Metabolic Longevity Protocol
Advanced metabolic longevity protocol combining SGLT2 inhibitor (dapagliflozin), Akkermansia muciniphila, and Berberine for comprehensive cardiometabolic optimisation. Built on the 2025 Cell Reports Medicine telomere data and 2024 Nature senolytic findings.
4 steps · 4 compounds
View protocol →The Skin-Longevity Protocol — Collagen, Astaxanthin, HA & GHK-Cu
A four-mechanism skin-longevity stack targeting the major addressable drivers of skin ageing: collagen peptides build and signal new dermal matrix, astaxanthin protects it from UV/ROS-driven MMP breakdown, oral hyaluronic acid restores dermal hydration, and topical GHK-Cu signals remodelling. Three oral compounds plus one topical, layered on top of daily sun protection. Designed for evidence-led skin elasticity, hydration, and wrinkle support over a 12-week horizon.
4 steps · 4 compounds
View protocol →The Stress Resilience Protocol — Rhodiola, Holy Basil & Ashwagandha
A layered adaptogen protocol that matches each herb's direction to the daily cortisol rhythm: rhodiola in the morning to activate and clear mental fatigue, holy basil through the day as a gentle metabolic base, and ashwagandha in the evening to lower cortisol and support sleep. Built to restore the natural shape of the stress curve — high cortisol in the morning, low at night — rather than blunting it indiscriminately. Start with the single herb that matches your worst time of day and build from there. A modest, low-risk margin on top of sleep, daylight, movement, and breathing.
3 steps · 3 compounds
View protocol →The Wolverine Stack — Elite Recovery Protocol
The most powerful peptide protocol for soft tissue injury recovery. Combines BPC-157's targeted local healing with TB-500's systemic cell mobilisation. Named for its near-miraculous regenerative capacity. Used by athletes, practitioners, and biohackers for tendon, ligament, and muscle repair.
3 steps · 2 compounds
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