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Endogenous Tripeptide / Master Antioxidant

Glutathione

The body's master antioxidant — a tripeptide of cysteine, glycine, and glutamate that every cell makes for itself. Levels fall with age, but the honest question is not whether glutathione matters; it is whether swallowing it does anything that supplying its precursors doesn't do better and cheaper.

longevitycellular-repairimmune-supportanti-inflammatory
Tier AWell-tolerated — strong human evidence
Evidence gradeBControlled trials / Cohort studies
BH

Reviewed & fact-checked by

BiohackingHub Research Team

Editorial Research Team · Last updated: July 31, 2026

Verified

What Is Glutathione?

Glutathione is a tripeptide — cysteine, glycine, glutamate — synthesised inside virtually every cell. It is the dominant intracellular antioxidant and the workhorse of phase II detoxification, the conjugation step where the liver tags fat-soluble toxins for excretion. Concentrations are highest in the liver, which is exactly where you would expect the body's chemical processing plant to keep its reagents.

Levels decline with age, and low glutathione tracks with oxidative stress and mitochondrial dysfunction across a large body of gerontology literature. That association is solid. What follows from it is where supplements get interesting.

Does Oral Glutathione Work?

The old objection was that glutathione is digested to its constituent amino acids before absorption, making supplementation pointless. That objection turned out to be too strong. A six-month randomised controlled trial found that oral glutathione raised body stores in blood, erythrocytes, and buccal cells at 250 and 1000 mg/day — a genuine, measured increase rather than a marketing claim.

So it works. The harder question is whether it works better than the alternative. The trials that produced the most impressive downstream outcomes — oxidative stress, mitochondrial function, inflammation, physical function in older adults — used precursors (glycine plus NAC), not intact glutathione. Precursor loading is also substantially cheaper.

Forms

FormNotes
Reduced (GSH)The form used in the positive RCT; standard and inexpensive
LiposomalPhospholipid encapsulation aims to protect the molecule through digestion; promising uptake data, thinner outcome data
S-acetylAcetylated for stability; mechanistically sensible, human evidence limited
Nebulised/IVClinical settings only — not a home intervention

Dosage

ParameterRecommendation
Low250 mg/day
Typical500 mg/day
High1000 mg/day
TimingOnce daily; liposomal forms away from food
AlternativeNAC 1200 mg + glycine — better evidence per dollar

Safety

Glutathione is what your cells already run on, and supplemental doses are well tolerated. There are no meaningful contraindications at these amounts; the caution worth flagging is for nebulised forms, which can provoke bronchospasm in asthmatics. Long-term high-dose data in healthy adults are still limited, which argues for staying near the studied range rather than escalating.

Related Research

Stacking Interactions

How Glutathione interacts with other compounds

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Synergistic

NAC supplies the rate-limiting cysteine — the cheaper and better-evidenced route to raising glutathione

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Synergistic

The second precursor amino acid; older adults are frequently short of both glycine and cysteine

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Synergistic

Silymarin supports hepatic glutathione status alongside its antifibrotic effects

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Synergistic

Vitamin C regenerates oxidised glutathione back to the reduced form — the classic redox recycling pair

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Synergistic

Glutathione peroxidases are selenoenzymes; without selenium, glutathione has fewer working enzymes to serve

Safety Profile — Tier A

Well-tolerated — strong human evidence

Contraindications

  • No absolute contraindications at supplemental doses
  • Asthma — caution with inhaled/nebulised forms, which can trigger bronchospasm

Side Effects

  • Well tolerated orally; occasional bloating or loose stools
  • Sulphurous taste or odour with some forms
  • Long-term high-dose data in healthy adults remain limited