Foundational Longevity Stack
The most widely adopted evidence-based longevity stack, built around NAD+ restoration and sirtuin pathway activation. Suitable for adults over 35 seeking a science-backed starting point for longevity supplementation.
Daily Schedule
Timing and dosage for each step
07:00 AM
500 mg
Take fasted or with a light breakfast. Morning timing aligns with peak NAMPT enzyme activity.
07:00 AM
500 mg
Take with a fat-containing meal (avocado, olive oil) to maximise absorption — increases bioavailability up to 5-fold.
07:00 AM
1000 mg
Take alongside NMN to support the methylation cycle and prevent methyl group depletion.
Overview
This protocol is the most widely referenced starting point for longevity supplementation, popularised by Dr David Sinclair's research at Harvard Medical School. It combines three compounds with complementary mechanisms:
- NMN restores declining NAD+ levels, enabling robust mitochondrial function and DNA repair
- Resveratrol activates SIRT1, the longevity-associated deacetylase that requires NAD+ — making it directly synergistic with NMN
- TMG donates methyl groups to prevent depletion during NMN metabolism
Morning Schedule
Take all three compounds together in the morning. The combined timing ensures:
- NMN and Resveratrol are both present when SIRT1 is most active (NAMPT enzyme peaks in the morning)
- Fat co-ingestion with Resveratrol (from breakfast) dramatically improves bioavailability
- TMG is available throughout the day to support the methylation cycle
Who This Protocol Is For
A beginner-friendly protocol suitable for healthy adults over 35 with no significant medical conditions. All compounds are available as OTC dietary supplements in the US and most EU countries.
Expected Timeline
Most users report subjective energy improvements within 2–4 weeks. Objective biomarkers (NAD+ blood levels) can be tested via home blood tests at 8–12 weeks to confirm response.
Important Notes
- Introduce compounds one at a time if you have known sensitivities
- Consult a physician if you take prescription medications, particularly blood thinners or immunosuppressants
- For research and educational purposes only — see our Medical Disclaimer